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蛍光活性化細胞選別-放射性リガンド処理組織(FACS-RTT)による放射性シグナルの細胞起源の決定
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JoVE Journal Neuroscience
Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal
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蛍光活性化細胞選別-放射性リガンド処理組織(FACS-RTT)による放射性シグナルの細胞起源の決定

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2,192 Views

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12:04 min

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September 10, 2021

DOI:

10.3791/62883-v

DOI:
10.3791/62883-v

•

12:04 min
September 10, 2021

•

2 Views
, , , ,
1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London

Summary

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蛍光活性化細胞選別 - 放射性リガンド処理組織(FACS-RTT)は、アルツハイマー病における18 kDaトランスロケータータンパク質またはセロトニン5HT2A受容体発現の役割を細胞規模で研究するための強力なツールです。このプロトコールは、TgF344−ADラットモデルにおけるFACS−RTTの エクスビボ 適用を記載する。

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Cite this Article

Amossé, Q., Ceyzériat, K., Tsartsalis, S., Tournier, B. B., Millet, P. Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal. J. Vis. Exp. (175), e62883, doi:10.3791/62883 (2021).

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