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Fluorescência-Ativada Célula Desarmamento-Radiolige Tecido Tratado (FACS-RTT) para determinar a origem celular do sinal radioativo
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September 10, 2021
DOI:
1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
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Fluorescência-Ativada Célula-Radioliging tecido tratado (FACS-RTT) é uma ferramenta poderosa para estudar o papel da proteína translocador de 18 kDa ou expressão de receptor de serotonina 5HT2A na doença de Alzheimer em escala celular. Este protocolo descreve a aplicação ex-vivo do FACS-RTT no modelo de rato TgF344-AD.
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Amossé, Q., Ceyzériat, K., Tsartsalis, S., Tournier, B. B., Millet, P. Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal. J. Vis. Exp. (175), e62883, doi:10.3791/62883 (2021).
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