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Un modèle sphéroïde tridimensionnel pour étudier l’interaction tumeur-stromale dans le carcinome hépatocellulaire
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September 30, 2021
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1Department of Biochemistry, Faculty of Pharmacy,Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy,University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences,Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences,Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital,Newcastle-upon-Tyne Hospitals NHS Foundation Trust
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Il manque des modèles in vitro complets qui récapitulent fidèlement la maladie humaine pertinente. La présente étude présente la création et la culture de sphéroïdes tumoraux tridimensionnels (3D), un outil in vitro fiable pour étudier l’interaction tumeur-stromale dans le carcinome hépatocellulaire humain.
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Zaki, M. Y. W., Shetty, S., Wilkinson, A. L., Patten, D. A., Oakley, F., Reeves, H. A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma. J. Vis. Exp. (175), e62868, doi:10.3791/62868 (2021).
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