Journal
/
Neuroscience
/
肌萎缩性侧索硬化症模型中突触功能的实时荧光测量
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
Please note that all translations are automatically generated. Click here for the English version.

肌萎缩性侧索硬化症模型中突触功能的实时荧光测量

DOI:
10.3791/62813-v

•

08:59 min

•

July 16, 2021

•

, , ,
1Jefferson Weinberg ALS Center,Thomas Jefferson University

Chapters

  • 00:04Introduction
  • 00:52Microscope and Perfusion System Setup
  • 02:33Styryl Dye Imaging of Synaptic Vesicle Release
  • 04:30Fluorescence Imaging of Gcamp6m Calcium Transients
  • 05:36Image Analysis
  • 06:23Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

描述了两种相关方法，以可视化突触传递所需的亚细胞事件。这些方案能够使用 体外 培养神经元的活细胞成像实时监测突触前钙流入和突触囊泡膜融合的动态。

Tags

Real-timeFluorescentSynapticAmyotrophic Lateral SclerosisSynaptic FunctionElectrophysiologyPrimary Rodent Neuronal CulturesInduced Pluripotent Stem CellsGCaMPStyryl DyeACSFPotassium ChlorideConfocal Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白

同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作

星形细胞转运电流派生谷氨酸间隙的时间进程与反卷积分析

初级量子神经元轴突运输的实时成像点标记的BDNF

实时 fMRI 神经训练管理协议

实时 cAMP 检测气味受体激活的活细胞测量

光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

气相臭受体活化的实时体外监测

多发性硬化患者综合解剖计划

正常小鼠和亨廷顿小鼠急性脑切片中谷氨酸释放和服用的单突触指标

Read Article