/
/
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
2,350 Views
•
08:59 min
•
July 16, 2021
DOI:
Summary
Automatically generated
Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.
Read Article
Cite this Article
Krishnamurthy, K., Trotti, D., Pasinelli, P., Jensen, B. Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (173), e62813, doi:10.3791/62813 (2021).
Copy