Journal
/
Neuroscience
/
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

English

Automatically Generated

2,349 Views

•

08:59 min

•

July 16, 2021

DOI:

10.3791/62813-v

DOI:
10.3791/62813-v

•

08:59 min
July 16, 2021

•

1 Views
, , ,
1Jefferson Weinberg ALS Center,Thomas Jefferson University

Summary

Automatically generated

Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.

Explore More Videos

Real-timeFluorescentSynapticAmyotrophic Lateral SclerosisSynaptic FunctionElectrophysiologyPrimary Rodent Neuronal CulturesInduced Pluripotent Stem CellsGCaMPStyryl DyeACSFPotassium ChlorideConfocal Imaging

Read Article

Cite this Article

Krishnamurthy, K., Trotti, D., Pasinelli, P., Jensen, B. Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (173), e62813, doi:10.3791/62813 (2021).

Download .ris file

Copy

Share Video

.

Copy