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Studi di interazioni Chaperone-Cochaperone utilizzando il saggio omogeneo basato su perle
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Biochemistry
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JoVE Journal Biochemistry
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
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Studi di interazioni Chaperone-Cochaperone utilizzando il saggio omogeneo basato su perle

italiano

Automatically Generated

2,560 Views

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06:51 min

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July 21, 2021

DOI:

10.3791/62762-v

DOI:
10.3791/62762-v

•

06:51 min
July 21, 2021

•

5 Views
, , , ,
1Department of Neuroscience Care and Society, Division of Neurogeriatrics,Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology,Indian Institute of Technology (BHU), 3Theme Inflammation and Aging,Karolinska University Hospital

Summary

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Questo protocollo presenta una tecnica per sondare le interazioni proteina-proteina utilizzando perle donatrici legate al glutatione con co-chaperoni tPR fusi GST e perle accettori accoppiate con un peptide derivato da Hsp90. Abbiamo usato questa tecnica per lo screening di piccole molecole per interrompere le interazioni Hsp90-FKBP51 o Hsp90-FKBP52 e identificato inibitori di interazione Hsp90-FKBP51 potenti e selettivi.

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Wang, L., Bergkvist, L., Kumar, R., Winblad, B., Pavlov, P. F. Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay. J. Vis. Exp. (173), e62762, doi:10.3791/62762 (2021).

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