/
/
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
2,560 Views
•
06:51 min
•
July 21, 2021
DOI:
Summary
Automatically generated
This protocol presents a technique for probing protein-protein interactions using glutathione-linked donor beads with GST-fused TPR-motif co-chaperones and acceptor beads coupled with an Hsp90-derived peptide. We have used this technique to screen small molecules to disrupt Hsp90-FKBP51 or Hsp90-FKBP52 interactions and identified potent and selective Hsp90-FKBP51 interaction inhibitors.
Read Article
Cite this Article
Wang, L., Bergkvist, L., Kumar, R., Winblad, B., Pavlov, P. F. Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay. J. Vis. Exp. (173), e62762, doi:10.3791/62762 (2021).
Copy