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一种优化的O9-1/水凝胶系统,用于研究神经嵴细胞中的机械信号
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JoVE Journal
Developmental Biology
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种优化的O9-1/水凝胶系统,用于研究神经嵴细胞中的机械信号
DOI:
10.3791/62693-v
•
11:02 min
•
August 13, 2021
•
Tram P. Le*
1
,
Xiaolei Zhao*
1
,
Shannon Erhardt
2
,
Jianhua Gu
,
Huie Wang
,
Tina O. Findley
,
Jun Wang
2
1
Department of Pediatrics, McGovern Medical School
,
The University of Texas Health Science Center at Houston
,
2
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
,
The University of Texas Health Science Center at Houston
,
3
SEM AFM Core in the Houston Methodist Hospital Research Institute
Chapters
00:05
Introduction
00:34
Preparation of Aminosilane-Coated and Siliconized Glass Coverslips
03:13
Preparation of Hydrogels
06:40
Molecular Analysis of Stiffness via Immunofluorescence Staining
08:04
Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System
10:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里描述了详细的分步方案,用于使用多能O9-1神经嵴细胞和不同硬度的聚丙烯酰胺水凝胶
在体外
研究机械信号。
Tags
Neural Crest Cells
Mechanical Signals
Chemical Signals
Neural Crest Development
Diseases
Glass Cover Slips
Sterilization
Sodium Hydroxide
Aminopropyl Triethoxysilane
Glutaraldehyde
Dichloromethane Silane
Hydrogel
Acrylamide Gel
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