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一种优化的O9-1/水凝胶系统,用于研究神经嵴细胞中的机械信号
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
DOI:

11:02 min

August 13, 2021

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Chapters

  • 00:05Introduction
  • 00:34Preparation of Aminosilane-Coated and Siliconized Glass Coverslips
  • 03:13Preparation of Hydrogels
  • 06:40Molecular Analysis of Stiffness via Immunofluorescence Staining
  • 08:04Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System
  • 10:31Conclusion

Summary

Automatic Translation

这里描述了详细的分步方案,用于使用多能O9-1神经嵴细胞和不同硬度的聚丙烯酰胺水凝胶 在体外 研究机械信号。

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