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Modelos 2D y 3D basados en células madre pluripotentes inducidas por humanos para diseccionar la participación del cilio primario durante el desarrollo neocortical
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Developmental Biology
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JoVE Journal Developmental Biology
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
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Modelos 2D y 3D basados en células madre pluripotentes inducidas por humanos para diseccionar la participación del cilio primario durante el desarrollo neocortical

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3,592 Views

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14:19 min

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March 25, 2022

DOI:

10.3791/62667-v

DOI:
10.3791/62667-v

•

14:19 min
March 25, 2022

•

17 Views
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1Imagine Institute, INSERM UMR 1163,Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology,APHP- Necker Enfants Malades Hospital

Summary

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Presentamos protocolos detallados para la generación y caracterización de modelos de desarrollo neocortical basados en células madre pluripotentes inducidas humanas (hIPSC) 2D y 3D, así como metodologías complementarias que permiten el análisis cualitativo y cuantitativo de la biogénesis y función del cilio primario (PC).

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Boutaud, L., Michael, M., Banal, C., Calderon, D., Farcy, S., Pernelle, J., Goudin, N., Maillard, C., Dimartino, C., Deleschaux, C., Dupichaud, S., Lebreton, C., Saunier, S., Attié-Bitach, T., Bahi-Buisson, N., Lefort, N., Thomas, S. 2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development. J. Vis. Exp. (181), e62667, doi:10.3791/62667 (2022).

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