Dergi
/
Gelişim Biyolojisi
/
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
JoVE Journal
Gelişim Biyolojisi
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

3,592 Views

•

14:19 min

•

March 25, 2022

DOI:

10.3791/62667-v

DOI:
10.3791/62667-v

•

14:19 min
March 25, 2022

•

17 Views
, , , , , , , , , , , , , , , ,
1Imagine Institute, INSERM UMR 1163,Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology,APHP- Necker Enfants Malades Hospital

Özet

Automatically generated

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.

Explore More Videos

2D3DHumanInduced Pluripotent Stem CellPrimary CiliumNeocortical DevelopmentNeural RosettesDorsal Forebrain OrganoidsEmbryoid BodyDual SMAD InhibitionImmunohistochemistryVibratomeConfocal Microscopy

Read Article

Cite this Article

Boutaud, L., Michael, M., Banal, C., Calderon, D., Farcy, S., Pernelle, J., Goudin, N., Maillard, C., Dimartino, C., Deleschaux, C., Dupichaud, S., Lebreton, C., Saunier, S., Attié-Bitach, T., Bahi-Buisson, N., Lefort, N., Thomas, S. 2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development. J. Vis. Exp. (181), e62667, doi:10.3791/62667 (2022).

Download .ris file

Copy

Share Video

.

Copy