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JoVE Journal Developmental Biology
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
 

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Article DOI: 10.3791/62667-v • 14:19 min • March 25th, 2022
March 25th, 2022 •
*1, *1, 2, 1, 1, 1, 3, 1, 1, 1, 4, 1, 1, 1,5, 1,6, 2, 1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
* These authors contributed equally

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We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.

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Keywords: 2D 3D Human Induced Pluripotent Stem Cell Primary Cilium Neocortical Development Neural Rosettes Dorsal Forebrain Organoids Embryoid Body Dual SMAD Inhibition Immunohistochemistry Vibratome Confocal Microscopy
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