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Journal
/
Genetics
/
冈
比亚按蚊
胚胎的显微注射方法
JoVE Journal
Genetics
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JoVE Journal
Genetics
Microinjection Method for
Anopheles gambiae
Embryos
Please note that all translations are automatically generated.
Click here for the English version.
冈
比亚按蚊
胚胎的显微注射方法
DOI:
10.3791/62591-v
•
05:39 min
•
July 07, 2021
•
Rebeca Carballar-Lejarazú
,
Taylor Tushar
,
Thai Binh Pham
,
Anthony A. James
2
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
Chapters
00:05
Introduction
00:51
Embryo Preparation
01:53
Embryo Alignment
02:35
Embryo Microinjection
03:53
Results: Microinjection Method to Target
Agcd
Gene Ortholog Using pCO37 Gene Drive Construct
05:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
显微注射技术对于将外源基因引入蚊子的基因组至关重要。该协议解释了詹姆斯实验室使用的一种方法,该方法将DNA构建体微注射到
冈比亚按蚊
胚胎中以产生转化的蚊子。
Tags
Microinjection
Anopheles Gambiae
Embryos
Transgenic
Malaria
Population Suppression
Mosquito Species
Aspirator
Conical Tube
Dental Film
Nylon Mesh
Filter Paper
Incubator
Forceps
Petri Dish
Membrane
Filter Paper
Microloader Tip
Needle Holder
Automated Pressure Pump
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