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Microinjection Method for Anopheles gambiae Embryos
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JoVE Journal Genética
Microinjection Method for Anopheles gambiae Embryos

Microinjection Method for Anopheles gambiae Embryos

4,173 Views

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05:39 min

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July 07, 2021

DOI:

10.3791/62591-v

DOI:
10.3791/62591-v

•

05:39 min
July 07, 2021

•

15 Views
, , ,
1Department of Microbiology & Molecular Genetics,University of California, Irvine, 2Department of Molecular Biology & Biochemistry,University of California, Irvine

Summary

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Microinjection techniques are essential to introduce exogenous genes into the genomes of mosquitoes. This protocol explains a method used by the James laboratory to microinject DNA constructs into Anopheles gambiae embryos to generate transformed mosquitoes.

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Carballar-Lejarazú, R., Tushar, T., Pham, T. B., James, A. A. Microinjection Method for Anopheles gambiae Embryos. J. Vis. Exp. (173), e62591, doi:10.3791/62591 (2021).

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