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Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
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JoVE Journal Genetics
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

English

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5,261 Views

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06:29 min

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April 15, 2021

DOI:

10.3791/62131-v

DOI:
10.3791/62131-v

•

06:29 min
April 15, 2021

•

14 Views
, , , , , ,
1Department of Vector Biology,Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology,University of Crete

Summary

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The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.

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GAL4-UAS SystemAnopheles GambiaeFunctional GeneticsGenetic ModificationGene ExpressionPhenotypic CharacterizationInsecticide ResistancePathogen TransmissionGenetic ControlPupae CollectionFluorescent MarkerExternal GenitaliaSexingAdult TransferBlood FeedingOviposition

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Cite this Article

Poulton, B. C., Colman, F., Anthousi, A., Grigoraki, L., Adolfi, A., Lynd, A., Lycett, G. J. Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae. J. Vis. Exp. (170), e62131, doi:10.3791/62131 (2021).

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