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Journal
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Biochemistry
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光学镊子研究翻译调控中的RNA-蛋白质相互作用
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Biochemistry
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JoVE Journal
Biochemistry
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Please note that all translations are automatically generated.
Click here for the English version.
光学镊子研究翻译调控中的RNA-蛋白质相互作用
DOI:
10.3791/62589-v
•
12:26 min
•
February 12, 2022
•
Lukas Pekarek
,
Stefan Buck
,
Neva Caliskan
2
1
Helmholtz Institute for RNA-based Infection Research (HIRI)
,
Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research)
,
2
Medical Faculty
,
Julius-Maximilians University Würzburg
Chapters
00:02
Introduction
01:56
Sample Preparation
04:20
Instrument Setup
05:47
Sample Measurement
09:19
Data Analysis
10:06
Representative Results
12:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议为使用光学镊子研究RNA-蛋白质相互作用提供了完整的实验工作流程。概述了几种可能的实验设置,包括光学镊子与共聚焦显微镜的组合。
Tags
Optical Tweezers
RNA-protein Interactions
Translation Regulation
Single Molecule Techniques
Fluorescence Assisted Optical Tweezers
Force-ramp Experiments
Constant Force Measurements
Real-time Visualization
DNA-RNA Construct
In-vitro Transcription
PCR Reactions
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