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Quantitative Immunofluorescence to Measure Global Localized Translation
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Biochemistry
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JoVE Journal Biochemistry
Quantitative Immunofluorescence to Measure Global Localized Translation

Quantitative Immunofluorescence to Measure Global Localized Translation

English

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9,610 Views

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09:13 min

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August 22, 2017

DOI:

10.3791/55909-v

DOI:
10.3791/55909-v

•

09:13 min
August 22, 2017

•

21 Views
,
1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie,Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec

Summary

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This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.

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Cite this Article

Bergeman, J., Huot, M. Quantitative Immunofluorescence to Measure Global Localized Translation. J. Vis. Exp. (126), e55909, doi:10.3791/55909 (2017).

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