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Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

English

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4,797 Views

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08:02 min

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May 14, 2021

DOI:

10.3791/62504-v

DOI:
10.3791/62504-v

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08:02 min
May 14, 2021

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12 Views
, , , , , ,
1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”,Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

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This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. 

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Truffi, M., Sitia, L., Sevieri, M., Bonizzi, A., Rizzuto, M. A., Mazzucchelli, S., Corsi, F. Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles. J. Vis. Exp. (171), e62504, doi:10.3791/62504 (2021).

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