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Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

DOI:
10.3791/62504-v

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08:02 min

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May 14, 2021

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, , , , , ,
1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”,Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

Chapters

  • 00:04Introduction
  • 00:37Tumor Dissociation into Single Cells
  • 02:55Extraction of Cancer-Associated Fibroblasts (CAFs) from Dissociated Tumor
  • 05:20Results-I: Isolation and Culture of Primary CAFs from a Syngeneic Murine Breast Cancer
  • 06:40Results-II: Use of Isolated CAFs to Evaluate Targeting Potential of Hfn-FAP Functionalized Nanodrugs
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. 

Tags

Keywords: Primary Cancer-associated FibroblastsMurine Breast CancerTumor MicroenvironmentIn Vitro StudiesTumor StromaTumor DissociationCell IsolationDead Cell RemovalMagnetic SeparationCell Culture

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