This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment.
Truffi, M., Sitia, L., Sevieri, M., Bonizzi, A., Rizzuto, M. A., Mazzucchelli, S., Corsi, F. Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles. J. Vis. Exp. (171), e62504, doi:10.3791/62504 (2021).