Journal
/
癌症研究
/
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
JoVE 杂志
癌症研究
This content is Free Access.
JoVE 杂志 癌症研究
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

4,797 Views

•

08:02 min

•

May 14, 2021

DOI:

10.3791/62504-v

DOI:
10.3791/62504-v

•

08:02 min
May 14, 2021

•

12 Views
, , , , , ,
1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”,Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

Summary

Automatically generated

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. 

Explore More Videos

Primary Cancer-associated FibroblastsMurine Breast CancerTumor MicroenvironmentIn Vitro StudiesTumor StromaTumor DissociationCell IsolationDead Cell RemovalMagnetic SeparationCell Culture

Read Article

Cite this Article

Truffi, M., Sitia, L., Sevieri, M., Bonizzi, A., Rizzuto, M. A., Mazzucchelli, S., Corsi, F. Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles. J. Vis. Exp. (171), e62504, doi:10.3791/62504 (2021).

Download .ris file

Copy

Share Video

.

Copy