Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Bearach Miwatani-Minter; Gergely Rona

doi:10.3791/62466

Journal

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Biology

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Microirradiación láser para estudiar el reclutamiento de ADN durante la fase S

JoVE Journal
Biology

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JoVE Journal Biology

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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Microirradiación láser para estudiar el reclutamiento de ADN durante la fase S

3,811 Views

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07:11 min

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April 16, 2021

DOI:

10.3791/62466-v

DOI:

10.3791/62466-v

•

07:11 min

April 16, 2021

•

19 Views

Bearach Miwatani-Minter^1,2, Gergely Rona^1,2,3

¹Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ²Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, ³Howard Hughes Medical Institute,New York University School of Medicine

Summary

Este protocolo describe un método no invasivo para identificar eficientemente las células de fase S para estudios de microscopía aguas abajo, como la medición del reclutamiento de proteínas de reparación del ADN mediante microirradiación láser.

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Microirradiación láser para estudiar el reclutamiento de ADN durante la fase S

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Summary

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