Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Bearach Miwatani-Minter; Gergely Rona

doi:10.3791/62466

Journal

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Biologie

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Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Journal JoVE
Biologie

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Journal JoVE Biologie

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

3,811 Views

•

07:11 min

•

April 16, 2021

DOI:

10.3791/62466-v

DOI:

10.3791/62466-v

•

07:11 min

April 16, 2021

•

19 Views

Bearach Miwatani-Minter^1,2, Gergely Rona^1,2,3

¹Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ²Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, ³Howard Hughes Medical Institute,New York University School of Medicine

Summary

This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser micro-irradiation.

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Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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