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Laser-Mikrobestrahlung zur Untersuchung der DNA-Rekrutierung während der S-Phase
JoVE Journal
Biology
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JoVE Journal Biology
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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Laser-Mikrobestrahlung zur Untersuchung der DNA-Rekrutierung während der S-Phase

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3,811 Views

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07:11 min

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April 16, 2021

DOI:

10.3791/62466-v

DOI:
10.3791/62466-v

•

07:11 min
April 16, 2021

•

19 Views
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1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, 3Howard Hughes Medical Institute,New York University School of Medicine

Summary

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Dieses Protokoll beschreibt eine nicht-invasive Methode zur effizienten Identifizierung von S-Phasen-Zellen für nachgelagerte Mikroskopiestudien, wie z. B. die Messung der Rekrutierung von DNA-Reparaturproteinen durch Lasermikrobestrahlung.

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Cite this Article

Miwatani-Minter, B., Rona, G. Laser Micro-Irradiation to Study DNA Recruitment During S Phase. J. Vis. Exp. (170), e62466, doi:10.3791/62466 (2021).

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