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Un método simplificado para generar organoides renales a partir de células madre pluripotentes humanas
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Developmental Biology
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JoVE Journal Developmental Biology
A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
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Un método simplificado para generar organoides renales a partir de células madre pluripotentes humanas

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4,210 Views

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07:39 min

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April 13, 2021

DOI:

10.3791/62452-v

DOI:
10.3791/62452-v

•

07:39 min
April 13, 2021

•

19 Views
, , , , ,
1Department of Developmental Biology,University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland, 3Center for Critical Care Nephrology,University of Pittsburgh, School of Medicine

Summary

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Aquí describimos un protocolo para generar organoides renales a partir de células madre pluripotentes humanas (hPSCs). Este protocolo genera organoides renales en dos semanas. Los organoides renales resultantes se pueden cultivar en matraces giratorios a gran escala o placas de agitación magnética de múltiples pozos para enfoques paralelos de prueba de drogas.

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Cite this Article

Przepiorski, A., Crunk, A. E., Holm, T. M., Sander, V., Davidson, A. J., Hukriede, N. A. A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells. J. Vis. Exp. (170), e62452, doi:10.3791/62452 (2021).

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