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Um método simplificado para gerar organoides renais a partir de células-tronco pluripotentes humanas
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Developmental Biology
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JoVE Journal Developmental Biology
A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

Um método simplificado para gerar organoides renais a partir de células-tronco pluripotentes humanas

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4,210 Views

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07:39 min

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April 13, 2021

DOI:

10.3791/62452-v

DOI:
10.3791/62452-v

•

07:39 min
April 13, 2021

•

19 Views
, , , , ,
1Department of Developmental Biology,University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland, 3Center for Critical Care Nephrology,University of Pittsburgh, School of Medicine

Summary

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Aqui descrevemos um protocolo para gerar organoides renais a partir de células-tronco pluripotentes humanas (hPSCs). Este protocolo gera organoides renais dentro de duas semanas. Os organoides renais resultantes podem ser cultivados em frascos rotadores em larga escala ou placas de agitação magnética multi-bem para abordagens paralelas de teste de drogas.

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Cite this Article

Przepiorski, A., Crunk, A. E., Holm, T. M., Sander, V., Davidson, A. J., Hukriede, N. A. A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells. J. Vis. Exp. (170), e62452, doi:10.3791/62452 (2021).

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