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A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

4,209 Views

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07:39 min

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April 13, 2021

DOI:

10.3791/62452-v

DOI:
10.3791/62452-v

•

07:39 min
April 13, 2021

•

19 Views
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1Department of Developmental Biology,University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland, 3Center for Critical Care Nephrology,University of Pittsburgh, School of Medicine

Summary

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Here we describe a protocol to generate kidney organoids from human pluripotent stem cells (hPSCs). This protocol generates kidney organoids within two weeks. The resulting kidney organoids can be cultured in large-scale spinner flasks or multi-well magnetic stir plates for parallel drug-testing approaches.

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Kidney OrganoidsHuman Pluripotent Stem CellsHPSCsCell CultureRenal DevelopmentRenal DiseaseSimplified MethodCell DifferentiationEmbryoid BodiesCell Culture Techniques

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Cite this Article

Przepiorski, A., Crunk, A. E., Holm, T. M., Sander, V., Davidson, A. J., Hukriede, N. A. A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells. J. Vis. Exp. (170), e62452, doi:10.3791/62452 (2021).

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