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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

English

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2,304 Views

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12:38 min

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November 06, 2021

DOI:

10.3791/62448-v

DOI:
10.3791/62448-v

•

12:38 min
November 06, 2021

•

8 Views
, ,
1Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,University of Alberta, 2School of Life Science and Engineering,Southwest Jiaotong University

Summary

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Techniques for generating a library of short peptides that can activate mast cells via the MRGPRX2 receptor are described. Associated techniques are easy, inexpensive, and can be extended to other cell receptors.

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Cite this Article

Raj, S., Lu, L., Unsworth, L. D. Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells. J. Vis. Exp. (177), e62448, doi:10.3791/62448 (2021).

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