Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Shammy Raj; Lei Lu; Larry  D. Unsworth

doi:10.3791/62448

Journal

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Immunologie et infection

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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

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Immunologie et infection
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Journal JoVE Immunologie et infection

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

2,304 Views

•

12:38 min

•

November 06, 2021

DOI:

10.3791/62448-v

DOI:

10.3791/62448-v

•

12:38 min

November 06, 2021

•

8 Views

Shammy Raj¹, Lei Lu², Larry D. Unsworth¹

¹Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,University of Alberta, ²School of Life Science and Engineering,Southwest Jiaotong University

Summary

Techniques for generating a library of short peptides that can activate mast cells via the MRGPRX2 receptor are described. Associated techniques are easy, inexpensive, and can be extended to other cell receptors.

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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Summary

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