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元素成像保护湿地根和岩石圈的方法
JoVE Journal
Environment
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JoVE Journal Environment
A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging
DOI:

06:29 min

February 15, 2021

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Chapters

  • 00:05Introduction
  • 00:43Slam-Freezing Equipment Preparation
  • 01:26Sample Collection and Slam-Freezing
  • 02:22Rhizosphere Cube Freeze-Drying and Embedding
  • 03:53Rhizosphere Cubes Trimming and Sectioning
  • 04:18Results: Elemental Wetland Root and Rhizosphere Preservation and Imaging
  • 05:53Conclusion

Summary

Automatic Translation

我们描述了一个协议,以水稻(Oryza水稻 L.)为模型物种,从湿地环境中取样、保存和分割完整的根部和周围的岩石圈土壤。保存后,可以使用元素成像技术(如同步加速器 X 射线荧光 (XRF) 化学光谱成像技术分析样品。

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