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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Neuroscience
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JoVE Journal Neuroscience
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

English

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6,082 Views

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07:19 min

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February 02, 2021

DOI:

10.3791/62215-v

DOI:
10.3791/62215-v

•

07:19 min
February 02, 2021

•

13 Views
, , ,
1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine,National Jewish Health, 2Section of Pediatric Gastroenterology, Hepatology, and Nutrition,Children’s Hospital Colorado

Summary

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Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

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Thomas, S. M., Bednarek, J., Janssen, W. J., Hume, P. S. Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation. J. Vis. Exp. (168), e62215, doi:10.3791/62215 (2021).

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