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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
Capítulos
- 00:05Introduction
- 00:43Air-Inflation Apparatus Preparation
- 01:40Animal Preparation
- 03:18Lung Air-Inflation, Perfusion, and Fixation
- 04:42Lung Extraction
- 05:42Results: Representative Lung Inflation, Extraction, and Histological Evaluation
- 06:47Conclusion
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
