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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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JoVE Journal 神経科学
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

DOI:
10.3791/62215-v

•

07:19 min

•

February 02, 2021

•

, , ,
1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine,National Jewish Health, 2Section of Pediatric Gastroenterology, Hepatology, and Nutrition,Children’s Hospital Colorado

章

  • 00:05Introduction
  • 00:43Air-Inflation Apparatus Preparation
  • 01:40Animal Preparation
  • 03:18Lung Air-Inflation, Perfusion, and Fixation
  • 04:42Lung Extraction
  • 05:42Results: Representative Lung Inflation, Extraction, and Histological Evaluation
  • 06:47Conclusion

概要

自動翻訳

自動翻訳

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

タグ

Air-inflationVascular Perfusion-fixationLung PreservationCell MorphologyAir Inflation ApparatusTracheaRight VentricleAbdominal WallDiaphragmThoracic CavitySternumTrachea IncisionLure Stub AdapterSuture

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