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Kromatin İmmünopresipitasyon Analizi için Dondurulmuş Kimerik Karaciğer Dokusundan Kromatin Ekstraksiyonu
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Biology
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JoVE Journal Biology
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
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Kromatin İmmünopresipitasyon Analizi için Dondurulmuş Kimerik Karaciğer Dokusundan Kromatin Ekstraksiyonu

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2,241 Views

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09:26 min

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March 23, 2021

DOI:

10.3791/62179-v

DOI:
10.3791/62179-v

•

09:26 min
March 23, 2021

•

7 Views
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1Department of Internal Medicine,University Medical Center Hamburg-Eppendorf, 2German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, 3Research Department Virus Immunology,Leibniz Institute for Experimental Virology

Summary

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Bu protokol, dondurulmuş dokulardan kromatin preparatına odaklanır ve Crosslinking Chromatin Immunoprecipitation (X-ChIP) ve ardından kantitatif PCR analizi (X-ChIP-qPCR) veya yeni nesil dizileme yaklaşımları (X-ChIP-seq) için uygundur.

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Pirosu, A., Allweiss, L., Dandri, M. Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis. J. Vis. Exp. (169), e62179, doi:10.3791/62179 (2021).

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