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Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
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Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

DOI:
10.3791/62179-v

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09:26 min

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March 23, 2021

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, ,
1İç Hastalıkları Anabilim Dalı,University Medical Center Hamburg-Eppendorf, 2German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, 3Research Department Virus Immunology,Leibniz Institute for Experimental Virology

Bölümler

  • 00:04Introduction
  • 00:26Tissue Crosslinking
  • 02:24Chromatin Isolation
  • 03:26DNA Purification
  • 06:50Results: ChIP‐qPCR and ChIP‐Seq Analysis
  • 08:52Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol focuses on chromatin preparation from snap frozen tissues and it is suitable for Crosslinking Chromatin Immunoprecipitation (X-ChIP) followed by either quantitative PCR analysis (X-ChIP-qPCR) or next generation sequencing approaches (X-ChIP-seq).

Etiketler

Chromatin ExtractionChimeric LiverChromatin ImmunoprecipitationPBSHomogenizerCentrifugationFormaldehydeGlycineBuffer ABuffer B

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