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クロマチン免疫沈降分析のための凍結キメラ肝臓組織からのクロマチン抽出
JoVE Journal
Biology
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JoVE Journal Biology
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
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クロマチン免疫沈降分析のための凍結キメラ肝臓組織からのクロマチン抽出

日本語

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2,241 Views

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09:26 min

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March 23, 2021

DOI:

10.3791/62179-v

DOI:
10.3791/62179-v

•

09:26 min
March 23, 2021

•

7 Views
, ,
1Department of Internal Medicine,University Medical Center Hamburg-Eppendorf, 2German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, 3Research Department Virus Immunology,Leibniz Institute for Experimental Virology

Summary

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このプロトコルは、スナップ凍結組織からのクロマチン調製に焦点を当てており、架橋クロマチン免疫沈降(X-ChIP)とそれに続く定量的PCR分析(X-ChIP-qPCR)または次世代シーケンシングアプローチ(X-ChIP-seq)のいずれかに適しています。

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Cite this Article

Pirosu, A., Allweiss, L., Dandri, M. Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis. J. Vis. Exp. (169), e62179, doi:10.3791/62179 (2021).

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