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06:37 min
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July 01, 2021
DOI:
1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Summary
Automatically generated
Burada CRISPR/Cas9 genom düzenlemesi tarafından oluşturulan Helicoverpa armigera (Hübner) embriyo mikroenjeksiyonu ve nakavt mutant tanımlama protokolü sunulmaktadır. Mutant böcekler, in vivo'daki farklı genler arasında gen fonksiyonu ve etkileşiminin daha fazla araştırılmasına olanak tanır.
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Cite this Article
Ai, D., Wang, B., Fan, Z., Fu, Y., Yu, C., Wang, G. Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner). J. Vis. Exp. (173), e62068, doi:10.3791/62068 (2021).
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