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Embryo Microinjectie en Knockout Mutant Identificatie van CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
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Behavior
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JoVE Journal Behavior
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
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Embryo Microinjectie en Knockout Mutant Identificatie van CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Nederlands

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4,188 Views

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06:37 min

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July 01, 2021

DOI:

10.3791/62068-v

DOI:
10.3791/62068-v

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06:37 min
July 01, 2021

•

9 Views
, , , , ,
1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences

Summary

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Hier wordt een protocol gepresenteerd van Helicoverpa armigera (Hübner) embryo micro-injectie en knock-out mutant identificatie gemaakt door CRISPR / Cas9 genoombewerking. Gemuteerde insecten maken verder onderzoek naar genfunctie en interactie tussen verschillende genen in vivo mogelijk.

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Cite this Article

Ai, D., Wang, B., Fan, Z., Fu, Y., Yu, C., Wang, G. Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner). J. Vis. Exp. (173), e62068, doi:10.3791/62068 (2021).

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