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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
 

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Summary
Automatic Translation

Presented here is a protocol of Helicoverpa armigera (Hübner) embryo microinjection and knockout mutant identification created by CRISPR/Cas9 genome editing. Mutant insects enable further research of gene function and interaction among different genes in vivo.

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Embryo Microinjection Knockout Mutant Identification CRISPR/Cas9 Genome Editing Helicoverpa Armigera Cotton Bollworm Gene Function Gene Interaction High Specificity Hereditable SgRNA Targets CRISPR Online Website Guide Sites Five Prime Untranslated Region PAM Option Editing Efficiency Off-target Editing Embryo Preparation Healthy Individuals Net Cage Sugar Solution Gauze Rubber Band
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