Journal
/
Biochemistry
/
Solunum Sinsitial Virüsünün Virüse Özgü Nükleopapsidlerinin Üretimi ve Montajı
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Please note that all translations are automatically generated. Click here for the English version.

Solunum Sinsitial Virüsünün Virüse Özgü Nükleopapsidlerinin Üretimi ve Montajı

Türkçe

Automatically Generated

3,567 Views

•

09:08 min

•

July 27, 2021

DOI:

10.3791/62010-v

DOI:
10.3791/62010-v

•

09:08 min
July 27, 2021

•

1 Views
, , , ,
1Department of Biochemistry,Emory University School of Medicine

Summary

Automatically generated

Solunum sinsitial virüsünün (RSV) RNA sentezinin derinlemesine mekanistik analizi için, virüse özgü nükleokapsidlerin (NC) sonraki in vitro montajı için RNA içermeyen nükleoproteinin (N0)birlikte ifade edilmesi için refakatçi fosfoprotein (P) kullanma protokolünü rapor ediyoruz.

Explore More Videos

Virus-specific NucleocapsidsRespiratory Syncytial VirusRecombinant Viral Protein ExpressionRNA-free N ProteinLigation Independent CloningElectron MicroscopyBi-cistronic ConstructionProtein ExpressionChromatographyCobalt Gravity ColumnQ ColumnHPLCUV Detection

Read Article

Cite this Article

Gao, Y., Ogilvie, C., Raghavan, A., Von Hoffmann, C., Liang, B. Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus. J. Vis. Exp. (173), e62010, doi:10.3791/62010 (2021).

Download .ris file

Copy

Share Video

.

Copy