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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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Biochemistry
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JoVE Journal Biochemistry
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

English

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3,572 Views

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09:08 min

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July 27, 2021

DOI:

10.3791/62010-v

DOI:
10.3791/62010-v

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09:08 min
July 27, 2021

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, , , ,
1Department of Biochemistry,Emory University School of Medicine

Summary

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For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).

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Cite this Article

Gao, Y., Ogilvie, C., Raghavan, A., Von Hoffmann, C., Liang, B. Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus. J. Vis. Exp. (173), e62010, doi:10.3791/62010 (2021).

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