Dergi
/
Biyokimya
/
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

3,572 Views

•

09:08 min

•

July 27, 2021

DOI:

10.3791/62010-v

DOI:
10.3791/62010-v

•

09:08 min
July 27, 2021

•

1 Views
, , , ,
1Department of Biochemistry,Emory University School of Medicine

Özet

Automatically generated

For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).

Explore More Videos

Virus-specific NucleocapsidsRespiratory Syncytial VirusRecombinant Viral Protein ExpressionRNA-free N ProteinLigation Independent CloningElectron MicroscopyBi-cistronic ConstructionProtein ExpressionChromatographyCobalt Gravity ColumnQ ColumnHPLCUV Detection

Read Article

Cite this Article

Gao, Y., Ogilvie, C., Raghavan, A., Von Hoffmann, C., Liang, B. Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus. J. Vis. Exp. (173), e62010, doi:10.3791/62010 (2021).

Download .ris file

Copy

Share Video

.

Copy