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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Bölümler
- 00:04Introduction
- 01:10Protein Expression and Purification
- 04:12In Vitro Virus-Specific Nucleocapsid (NC) Assembly
- 05:19Negative Stain Grid Preparation
- 07:17Results: Representative N1-391P1-126 Complex Copurification
- 08:08Conclusion
For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).
