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Journal
/
Neuroscience
/
小鼠视网膜的经瞳双光子体内成像
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
Please note that all translations are automatically generated.
Click here for the English version.
小鼠视网膜的经瞳双光子体内成像
DOI:
10.3791/61970-v
•
09:03 min
•
February 13, 2021
•
Zelun Wang
2
,
Sean McCracken
2
,
Philip R. Williams
3,4
1
John F. Hardesty, MD Department of Ophthalmology and Visual Sciences
,
Washington University School of Medicine
,
2
Graduate Program in Neuroscience
,
Washington University School of Medicine
,
3
Department of Neuroscience
,
Washington University School of Medicine
,
4
Hope Center for Neurological Disorders
,
Washington University School of Medicine
Chapters
00:05
Introduction
00:42
Imaging Preparation
03:44
Two-Photon Image Acquisition
06:13
Mouse Recovery
06:46
Results: Representative In Vivo Mouse Retina Imaging
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
体内成像是研究健康和疾病生物学的有力工具。该协议描述了使用标准双光子显微镜对小鼠视网膜进行经瞳孔成像。它还展示了不同的活体成像方法,以荧光标记视网膜的多个细胞队列。
Tags
In Vivo Imaging
Two-photon Microscopy
Mouse Retina
Ophthalmological Diseases
Retinal Cells
Stabilization
Head Holder
Pupil Dilation
Ear Canal Pins
Bite Bar
Eye Lubricant
Fluorescence
Retinal Structures
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