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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, 2Graduate Program in Neuroscience,Washington University School of Medicine, 3Department of Neuroscience,Washington University School of Medicine, 4Hope Center for Neurological Disorders,Washington University School of Medicine
Kapitel
- 00:05Introduction
- 00:42Imaging Preparation
- 03:44Two-Photon Image Acquisition
- 06:13Mouse Recovery
- 06:46Results: Representative In Vivo Mouse Retina Imaging
- 08:36Conclusion
In vivo imaging is a powerful tool for the study of biology in health and disease. This protocol describes transpupillary imaging of the mouse retina with a standard two-photon microscope. It also demonstrates different in vivoimaging methods to fluorescently label multiple cellular cohorts of the retina.
