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Método de interferência de RNA mediado por eletroporação em Odonata
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Electroporation-mediated RNA Interference Method in Odonata

Método de interferência de RNA mediado por eletroporação em Odonata

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5,326 Views

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13:28 min

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February 06, 2021

DOI:

10.3791/61952-v

DOI:
10.3791/61952-v

•

13:28 min
February 06, 2021

•

3 Views
, ,
1Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 2Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences,University of Tsukuba

Summary

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Fornecemos um protocolo detalhado para a interferência mediada por eletroporação no RNA em insetos da ordem Odonata (libélulas e damselflies) usando a damselfly de cauda azul(Ischnura senegalensis: Coenagironidae: Zygoptera) e a libélula de skimmer pied(Pseudothemis zonata: Libellulidae: Anisoptera).

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Cite this Article

Okude, G., Fukatsu, T., Futahashi, R. Electroporation-mediated RNA Interference Method in Odonata. J. Vis. Exp. (168), e61952, doi:10.3791/61952 (2021).

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