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Electroporation-mediated RNA Interference Method in Odonata
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Electroporation-mediated RNA Interference Method in Odonata

Electroporation-mediated RNA Interference Method in Odonata

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13:28 min

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February 06, 2021

DOI:

10.3791/61952-v

DOI:
10.3791/61952-v

•

13:28 min
February 06, 2021

•

3 Views
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1Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 2Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences,University of Tsukuba

Summary

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We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).

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ElectroporationRNA InterferenceRNAiOdonataDragonflyDamselflyIschnura SenegalensisGene Function AnalysisIn VivoProthoraxAbdomenSiRNADsRNAElectroporator

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Cite this Article

Okude, G., Fukatsu, T., Futahashi, R. Electroporation-mediated RNA Interference Method in Odonata. J. Vis. Exp. (168), e61952, doi:10.3791/61952 (2021).

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