Rivista
/
Biologia dello sviluppo
/
Electroporation-mediated RNA Interference Method in Odonata
JoVE Journal
Biologia dello sviluppo
Author Produced
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Electroporation-mediated RNA Interference Method in Odonata

Electroporation-mediated RNA Interference Method in Odonata

5,326 Views

•

13:28 min

•

February 06, 2021

DOI:

10.3791/61952-v

DOI:
10.3791/61952-v

•

13:28 min
February 06, 2021

•

3 Views
, ,
1Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 2Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences,University of Tsukuba

Summary

Automatically generated

We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).

Explore More Videos

ElectroporationRNA InterferenceRNAiOdonataDragonflyDamselflyIschnura SenegalensisGene Function AnalysisIn VivoProthoraxAbdomenSiRNADsRNAElectroporator

Read Article

Cite this Article

Okude, G., Fukatsu, T., Futahashi, R. Electroporation-mediated RNA Interference Method in Odonata. J. Vis. Exp. (168), e61952, doi:10.3791/61952 (2021).

Download .ris file

Copy

Share Video

.

Copy