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CRISPR/Cas9突然変異誘発のためのサンドフライ(フレボトムスパパタシ)胚微小注射

JoVE Journal
Genetics

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JoVE Journal Genetics

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

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CRISPR/Cas9突然変異誘発のためのサンドフライ(フレボトムスパパタシ)胚微小注射

6,674 Views

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05:44 min

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November 17, 2020

DOI:

10.3791/61924-v

DOI:

10.3791/61924-v

•

05:44 min

November 17, 2020

•

14 Views

Isabelle Louradour¹, Kashinath Ghosh¹, Ehud Inbar¹, David L. Sacks¹, Channa Aluvihare², Robert A. Harrell II²

¹Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ²University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Summary

このプロトコルは、胚の採取、注射、昆虫飼育、同定、および目的の突然変異の選択など、砂ハエにおけるCRISPR/Cas9標的突然変異のステップを詳述する。

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