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Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
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Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

6,655 Views

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05:44 min

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November 17, 2020

DOI:

10.3791/61924-v

DOI:
10.3791/61924-v

•

05:44 min
November 17, 2020

•

14 Views
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1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 2University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Summary

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This protocol details the steps of CRISPR/Cas9 targeted mutagenesis in sand flies: embryo collection, injection, insect rearing, and identification as well as selection of mutations of interest.

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Sand FlyPhlebotomus PapatasiEmbryo MicroinjectionCRISPR/Cas9MutagenesisInsect Genome EditingBlood FeedingEgg-layingFilter PaperMicroscopeInjection NeedlePneumatic Injection Controller

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Cite this Article

Louradour, I., Ghosh, K., Inbar, E., Sacks, D. L., Aluvihare, C., Harrell II, R. A. Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis. J. Vis. Exp. (165), e61924, doi:10.3791/61924 (2020).

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