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Sand Fly (Phlebotomus papatasi) Microiniezione embrionale per CRISPR/Cas9 Mutagenesi
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Genetics
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JoVE Journal Genetics
Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
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Sand Fly (Phlebotomus papatasi) Microiniezione embrionale per CRISPR/Cas9 Mutagenesi

italiano

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6,668 Views

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05:44 min

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November 17, 2020

DOI:

10.3791/61924-v

DOI:
10.3791/61924-v

•

05:44 min
November 17, 2020

•

14 Views
, , , , ,
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 2University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Summary

Automatically generated

Questo protocollo descrive in dettaglio le fasi della mutagenesi mirata CRISPR/Cas9 nelle mosche della sabbia: raccolta, iniezione, allevamento e identificazione degli insetti, nonché selezione di mutazioni di interesse.

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Cite this Article

Louradour, I., Ghosh, K., Inbar, E., Sacks, D. L., Aluvihare, C., Harrell II, R. A. Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis. J. Vis. Exp. (165), e61924, doi:10.3791/61924 (2020).

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