Fecal (micro) RNA Isolation

Fyonn H. Dhang; Howard L. Weiner; Shirong Liu

doi:10.3791/61908

Journal

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Immunology and Infection

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Aislamiento de (micro) ARN fecal

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Fecal (micro) RNA Isolation

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Aislamiento de (micro) ARN fecal

4,132 Views

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05:35 min

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October 28, 2020

DOI:

10.3791/61908-v

DOI:

10.3791/61908-v

•

05:35 min

October 28, 2020

•

42 Views

Fyonn H. Dhang^1,2, Howard L. Weiner^1,2, Shirong Liu^1,2

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, ²Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Summary

Este protocolo aísla ARN total de alta calidad de muestras fecales de sujetos animales y humanos. Se utiliza un kit de aislamiento de miARN comercial con una adaptación significativa para aislar ARN puro con cantidad y calidad optimizadas. Los aislados de ARN son buenos para la mayoría de los ensayos de ARN aguas abajo, como la secuenciación, la micromatriz y la RT-PCR.

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Aislamiento de (micro) ARN fecal

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Summary

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