Fecal (micro) RNA Isolation

Fyonn H. Dhang; Howard L. Weiner; Shirong Liu

doi:10.3791/61908

Journal

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Immunology and Infection

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Isolamento de RNA fecal (micro)

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Fecal (micro) RNA Isolation

Isolamento de RNA fecal (micro)

4,132 Views

•

05:35 min

•

October 28, 2020

DOI:

10.3791/61908-v

DOI:

10.3791/61908-v

•

05:35 min

October 28, 2020

•

42 Views

Fyonn H. Dhang^1,2, Howard L. Weiner^1,2, Shirong Liu^1,2

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, ²Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Summary

Este protocolo isola RNA total de alta qualidade de amostras fecais de animais e seres humanos. Um kit comercial de isolamento de miRNA é usado com adaptação significativa para isolar o RNA puro com quantidade e qualidade otimizadas. Os isolados de RNA são bons para a maioria dos ensaios de RNA a jusante, como sequenciamento, micro-array e RT-PCR.

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Isolamento de RNA fecal (micro)

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